Like other fibroblasts, cultured NIH-3T3 fibroblasts produce fibronectin in a constitutive fashion, and we have previously demonstrated that nicotine greatly enhances fibronectin expression in these cells. To examine the effects of FP on fibronectin protein expression, NIH-3T3 fibroblasts were pretreated in the presence or absence of FP for 1 h, cultured with or without nicotine (50 pg/mL) for 48 h, followed by harvesting and processing for western blotting. FP pretreatment […]